JHU biomedical engineering primary faculty
Warren L. Grayson, PhD
Associate ProfessorOffice: Smith 5023
Lab: Laboratory for Craniofacial and Orthopaedic Tissue Engineering
Postdoc, Biomedical Engineering, Columbia University
Ph.D., Biomedical Engineering, Florida State University, 2005
B.S., Chemical and Proc. Engineering, The University of the West Indies, Trinidad, 1998
Our lab seeks to address the challenges associated with engineering functional craniofacial and orthopaedic constructs for use in therapeutic applications. We are developing innovative methods using stem cells to create patient-specific grafts with the necessary biological and mechanical characteristics to facilitate functional in vivo integration.
We employ engineering techniques to design advanced bioreactors capable of maintaining cell viability in large tissue constructs.Bioreactors also enable precise control of the cellular microenvironment and can uniquely address fundamental questions regarding the application of biophysical cues to regulate stem cell differentiation, namely: How do the cells integrate multiple signals into decisions regarding cell fate? Can we regulate the spatial and temporal application of specific cues so that cells in different regions of the tissue constructs differentiate along diverse lineages simultaneously or sequentially? What signals are required to guide the hierarchical organization of these multiple cell types on various length scales?
Addressing the underlying mechanisms that regulate tissue development will enable us to engineer complex functional tissue constructs for regenerative medicine applications as well as to create relevant in vitro disease models.
Hutton DL, Moore EM, Gimble JM, Grayson WL. PDGF and Spatiotemporal Cues Induce Development of Vascularized Bone Tissue by Adipose-derived Stem Cells. Tissue Eng Part A. Accepted 2013.
Hutton DL, Logsdon EA, Moore EM, Gabhann FM, Gimble JM, Grayson WL. Vascular Morphogenesis of Adipose-Derived Stem Cells is Mediated by Heterotypic Cell-Cell Interactions. Tissue Eng Part A. 2012 Aug;18(15–16):1729-40. * Featured on the Cover *
Marolt D, Marcos Campos I, Bhumiratana S, Koren A, Petridis P, Zhang G, Spitalnik PF, Grayson WL, and Vunjak-Novakovic G. Engineering bone tissue from human embryonic stem cells. Proceedings of the National Academy of Sciences, 2012, 109 (22) 8705–8709.
Salter EK, Goh B, Hung B, Hutton DL, Ghone N, and Grayson WL. Bone Tissue Engineering Bioreactors: A Role in the Clinic? Tissue Eng Part B Rev. 2012 Feb;18(1):62–75.
Grayson WL, Frohlich M, Yeager K, Bhumiratana S, Canizzarro C, Wan LQ, Chan E, Liu X, Guo XE, Vunjak-Novakovic G. 2010. Engineering Anatomically-Shaped Human Mandibular Bone Grafts. Proceedings of the National Academy of Sciences: Special Issue in Regenerative Medicine,107(8): 3299–3304.