Warren L. Grayson, Ph.D. Assistant Professor, Biomedical Engineering Rangos 435
410-502-6306
wgrayso1 AT jhmi.edu EducationB.Sc., The University of the West Indies, Chemical & Process Engineering
Ph.D., Florida State University, Biomedical Engineering
Postdoc, Columbia University, Biomedical Engineering Research InterestsOur lab seeks to address the challenges associated with engineering functional craniofacial and orthopaedic constructs for use in therapeutic applications. We are developing innovative methods using stem cells to create patient-specific grafts with the necessary biological and mechanical characteristics to facilitate functional in vivo integration.
We employ engineering techniques to design advanced bioreactors capable of maintaining cell viability in large tissue constructs.Bioreactors also enable precise control of the cellular microenvironment and can uniquely address fundamental questions regarding the application of biophysical cues to regulate stem cell differentiation, namely: How do the cells integrate multiple signals into decisions regarding cell fate? Can we regulate the spatial and temporal application of specific cues so that cells in different regions of the tissue constructs differentiate along diverse lineages simultaneously or sequentially? What signals are required to guide the hierarchical organization of these multiple cell types on various length scales?
Addressing the underlying mechanisms that regulate tissue development will enable us to engineer complex functional tissue constructs for regenerative medicine applications as well as to create relevant in vitro disease models.
Selected PublicationsWL Grayson, M Fröhlich, K Yeager, S Bhumiratana, ME Chan, C Cannizzaro, LQ Wan, XS Liu, XE Guo and G Vunjak-Novakovic. 2009. Engineering Anatomically Shaped Human Bone Grafts, Proceedings of the National Academy of Sciences. In Press
T Ma, WL Grayson, M Fröhlich and G Vunjak-Novakovic. 2008. Hypoxia and Stem-Cell Based Engineering of Mesenchymal Tissues. Biotechnology Progress: Special Issue on Stem Cell Engineering, 25: 32 - 42
WL Grayson, S Bhumiratana, PHG Chao, C Cannizzaro, D Lennon, AI Caplan and G Vunjak-Novakovic. 2008. Increased Perfusion Rate and Cell-Seeding Density Enhance Tissue Engineering of Human Bone. Tissue Engineering (Part A), 14: 1809 - 1820
WL Grayson, PHG Chao, D Marolt, D Kaplan, and G Vunjak-Novakovic. 2008. Engineering Custom-Designed Osteochondral Grafts. Trends in Biotechnology, 26:181 - 189
WL Grayson, F Zhao, R Izadpanah, B Bunnell and T Ma. 2006. Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs, Journal of Cellular Physiology, 207: 331 - 339 Publications Search> From Pub Med > From Google Scholar  < Back to Primary Faculty page.
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