JHU biomedical engineering primary faculty
Leslie Tung, PhD
Professor and Interim Director
Professor of Biomedical Engineering
Interim Director, Department of Biomedical Engineering
Lab: Cardiac Bioelectric Systems Laboratory
B.S., M.S., Electrical Engineering, Massachusetts Institute of Technology, 1972
Ph.D., Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 1978
Engineering the Heart in a Dish
Our research involves the study of functional properties of engineered networks of living cardiac cells at varying structural levels that include single cells, cell strands, 2D sheets, and 3D tissue. We culture neonatal rat ventricular cells, human embyonic stem cell-derived cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes as multicellular networks to investigate fundamental properties of action potential propagation and arrhythmias. The use of voltage-sensitive dyes and optical mapping allows us to follow the temporal and spatial patterns of electrical activity.
Our work is currently directed within the following areas:
- Functional properties of cardiomyocytes derived from human pluripotent stem cells
- Engiineered cardiac tissues
- High frequency alternating current for cardiac therapy
- Myofibroblast-cardiomyocyte interactions More details are available on our lab website.
Blazeski, A., Zhu, R., Hunter, D. W., Weinberg, S. H., Zambidis, E. T., and Tung, L. Cardiomyocytes derived from human induced pluripotent stem cells as models for normal and diseased cardiac electrophysiology and contractility. Prog Biophys Mol Biol 110:166–177, 2012.
Weinberg, S. H., Chang, K. C., Zhu, R., Tandri, H., Berger, R. D., Trayanova, N. A., and Tung, L. Defibrillation success with high frequency electric fields is related to degree and location of conduction block. Heart Rhythm 10:740–748, 2013.
Joshi-Mukherjee, R., Dick, I. E., Liu, T., O'Rourke, B., Yue, D.T., and Tung, L. Structural and functional plasticity in long-term cultures of adult ventricular myocytes. J Mol Cell Cardiol 65:76–87, 2013.
Thompson, S. A., Blazeski, A., Copeland, C. R., Cohen, D. M., Chen, C. S., Reich, D. M., and Tung, L. Acute slowing of cardiac conduction in response to myofibroblast coupling to cardiomyocytes through N-cadherin. J Mol Cell Cardiol 68:29–37, 2014.