JHU biomedical engineering faculty
Andre Levchenko, Ph.D.
Professor of Biomedical Engineering
Office: Clark 208C
Lab: Signal Transduction and Cell-Cell Communication Lab
Eng.Sc.D., Bioengineering, Columbia University, 1992
M.S., Bioengineering, Columbia University, 1995
M.S., Biophysics, Moscow Institute of Physics and Technology, 1992
Intracellular Signal Transduction and Cell-Cell Communication
The advent of the New Biology characterized by high throughput technologies and recombinant DNA technologies and novel imaging techniques has revolutionized our approach to doing science. In particular, Systems Biology is developing by taking an integrated closed-loop approach, in which theory guides the way experiments are performed and experiments help refine theory. Our lab is taking this approach by combining molecular biology, microfabrication and imaging techniques and combining them with the state of the art modeling studies to investigate how living cells can sense their environment and how this sensing ability can help them establish communication leading to complex ensemble responses. We are studying, by modeling and experiment, MAPK, NF-?B, PKA and PI3K signal transduction pathways in eukaryotes. These pathways have been implicated in all vital cellular functions, such as cell cycle, locomotion and apoptosis, and various pathologies, including cancer and AIDS. We are also studying the ways simple organisms can display complex behavior in formation of biofilms and other superstructures implicated in over 65% of bacterial infections. In addition, we are exploring novel mechanisms of cell-cell communication involving physical transfer of cell membrane components between eukaryotic cells. Finally, we integrate the experimentally validated models and relate them to gene network regulation and other cell responses.
Levchenko, A., Bruck, J. and Sternberg, P.W. Scaffold proteins can alter multiple parameters of MAP kinase signaling modules. Proceedings of the National Academy of Science, 97(11):5818-23 (2000)
Levchenko, A. Computational cell biology in the post-genomic era. Molecular Biology Reports (in press)
Levchenko, A., Iglesias, P.A. Models of eukaryotic gradient sensing: application to chemotaxis of amoebae and neutrophils, Biophysical Journal 82: 50-63 (2002)
Levchenko, A., Bruck, J. and Sternberg, P.W. Combination of the biphasic response regulation and positive feedback as a general regulatory mechanism in homeostasis and signal transduction. In Foundations of Systems Biology, ed. H. Kitano, MIT Press, 2002
Shapiro, B.E., Levchenko, A. and Mjolsness, E. Automatic model generation for signal transduction with applications to MAPK pathway. In Foundations of Systems Biology, ed. H. Kitano, MIT Press, 2002.